Effects of Electronic Cigarette E-Liquids on the Adhesion and Growth of Osteoblast Cells

Electronic cigarettes (E-cigarettes) were designed to replace traditional decrease the harmful effects of smoking. The liquid in e-cigarettes contains different chemicals, such as Propylene Glycol (PG) and Vegetable Glycerin (VG), may contain nicotine. To be more attractive, e-liquids are supplemented with flavorings. Because vaped shown damaging oral tissues, similar could occur cavity non-vaped e-liquids. We evaluated e-liquid constituents on osteoblast behavior. For this purpose, human cells (MG-63) exposed containing 70% PG -30% VG without tobacco flavor or nicotine at 12, 18 mg/mL, no content. used various concentrations (0, 1, 5%). evaluate effect morphology adhesion, optical microscope observations viable cell counting using trypan blue exclusion used. Cell growth was also analyzed Methyl Thiazol Tetrazolium (MTT) assay, while cytotoxicity tested by measuring Lactate Dehydrogenase (LDH) levels after exposure for 24 h Our results show that induced significant morphological changes evidenced round forms, contact between cells. Adhesion significantly reduced, particularly presence E-liquids a concentration 5% reduced growth. This observed both flavored non-flavored decreased adhesion confirmed increased LDH. Overall indicate potentially chemicals bone cells, which lead impairment regeneration tissue remodeling processes.